Journal: Biomicrofluidics

Article Title: Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor

doi: 10.1063/5.0228901

Figure Lengend Snippet: Overview and RNA quality assessment of the microarray experiment. (a) Schematic workflow of the miRNA microarray experiment, created with BioRender.com . (b) Quality evaluation of total cellular RNA using the Agilent TapeStation 4200.

Article Snippet: Approximately 130 ng of biotin-tagged samples, along with hybridization controls, were loaded into GeneChip miRNA 4.0 microarray cartridges (array format type 100, Thermo Fisher Scientific, USA) and hybridized for 18 ± 0.5 h at 48 ° C with 60 rpm rotation in GeneChip Hybridization Oven 645.

Techniques: Microarray

Journal: Biomicrofluidics

Article Title: Impact of dcEF on microRNA profiles in glioblastoma and exosomes using a novel microfluidic bioreactor

doi: 10.1063/5.0228901

Figure Lengend Snippet: The Venn diagrams of miRNA microarray results from (a) cellular RNA and (b) exosomal RNA in three cell types after 100 V m − 1 dcEF stimulation.

Article Snippet: Approximately 130 ng of biotin-tagged samples, along with hybridization controls, were loaded into GeneChip miRNA 4.0 microarray cartridges (array format type 100, Thermo Fisher Scientific, USA) and hybridized for 18 ± 0.5 h at 48 ° C with 60 rpm rotation in GeneChip Hybridization Oven 645.

Techniques: Microarray

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 1. MPE miRNAs of lung adenocarcinoma patients. (A) The microarray detection results of miRNAs(Cluster Analysis). (B) The expression of miR- 93 and miR-146a in pleural effusion through real-time quantification RT-PCR. *P < 0.05. MPE, malignant pleural effusion.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Microarray, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 3. Effect of miR-93 on cell migration in HLEC and HUVEC. A duration of 24 h later, the cells that had migrated through the membrane were fixed with 95% alcohol and stained with crystal violet. The number of migrated cells was quantified by counting five independent symmetrical visual fields under the microscope (×100). The number of HLEC and HUVEC transfected with miR-93 mimic passing through the membrane was significantly lower than that of HLEC and HUVEC transfected with negative controls. (A) HLEC. (B) HUVEC. (a) blank control. (b) and (d) negative control (NC- miRNA). (c) miR93 mimic. (e) miR-93 inhibitor. Scare bar = 100 μm. *P < 0.05. HUVEC, human umbilical vein endothelial cells.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Migration, Membrane, Staining, Microscopy, Transfection, Control, Negative Control

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 2. PCR detection of miR-93 expression changes in cell lines after transient transfection. Expression levels of miR-93 in two cell lines were indicated. Cells were transfected with either 20 μmol/L of miR-93 mimic, miR-93 inhibitor, and NC-miRNA. A duration of 48 h after transfection, the expression of miR-93 was detected and normalized to internal GAPDH and U6 controls, respectively. (A) HLEC. (B) HUVEC. *P < 0.05. HUVEC, human umbilical vein endothelial cells.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Expressing, Transfection

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 5. Effect of miR-93 on cell proliferation in HLEC and HUVEC with clone formation assay. A statistically significant reduction in the number of colonies was observed in miR93 mimic group. (A) HLEC. (B) HUVEC. (a) blank control. (b) and (d) negative control (NC-miRNA). (c) miR93 mimic. (e) miR-93 inhibitor. Scare bar = 100 μm. *P < 0.05. HUVEC, human umbilical vein endothelial cells.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Tube Formation Assay, Control, Negative Control

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 6. Effect of miR-93 on cell tube formation capacity in HLEC and HUVEC with Matrigel plug assay. Matrigel assays were performed on HLEC and HUVEC 24 h after the transfection. Results are expressed as number of mean tube length and branching points. Branching points were counted by using MetaMorph. MiR-93 mimic significantly weakened the tube formation capacity of HLEC (A) and HUVEC (B). Branching points and mean tube length also decreased in MiR-93 mimic group (C,D). (A) HLEC. (B) HUVEC. (a) blank control. (b) and (d) negative control (NC-miRNA). (c) miR93 mimic. (e) miR-93 inhibitor. Scare bar = 50 μm. *P < 0.05. HUVEC, human umbilical vein endothelial cells.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Matrigel Assay, Transfection, Control, Negative Control

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 7. MiR-93 enhances apoptosis of epithelial cells. A duration of 48 h after the transfection with miRNA mimics, HLEC and HUVEC were collected for apoptosis analysis. The apoptotic rates were detected by flow cytometry (A, B). Results demonstrated the increased apoptosis in miR-93 mimic group. Results demonstrated the increased percentage of apoptosis cells in miR-93 mimic group. (A) HLEC. (B) HUVEC. (a) blank control. (b) and (d) NC-miRNA. (c) miR93 mimic. (e) miR-93 inhibitor. *P < 0.05. HUVEC, human umbilical vein endothelial cells. The apoptotic rates were detected by flow cytometry (A, B) and showed in histograms (C).

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Transfection, Flow Cytometry, Control

Journal: Cancer medicine

Article Title: The role of microRNA-93 regulating angiopoietin2 in the formation of malignant pleural effusion.

doi: 10.1002/cam4.1000

Figure Lengend Snippet: Figure 8. Cell cycle detection after miR-93 intervention. MiR-93 changes the cell cycle profile of HLEC and HUVEC. A duration of 48 h after the transfection with miRNA mimics, HLEC and HUVEC were collected and stained with PI. The cell cycle was detected by flow cytometry (A, B). Results demonstrated the increased percentage of G1 cells in miR-93 mimic group (C). (A) HLEC. (B) HUVEC. (a) blank control. (b) and (d) NC-miRNA. (c) miR93 mimic. (e) miR-93 inhibitor. *P < 0.05. HUVEC, human umbilical vein endothelial cells; PI, propidium iodide.

Article Snippet: According to the manufacturer’s instructions, we used the miRcute miRNA isolation kit (Tiangen, Beijing, China) to extract the enriched miRNA from fresh- frozen samples and cells.

Techniques: Transfection, Staining, Flow Cytometry, Control